Journal: International journal of food microbiology

Article Title: Emerging of Shiga toxin-producing Escherichia coli O177:H11 and O177:H25 from cattle at slaughter in Italy.

doi: 10.1016/j.ijfoodmicro.2024.110846

Figure Lengend Snippet: Fig. 1. Heatmap showing the presence/absence of virulence genes (y-axis) within the STEC isolates identified in this study (x-axis). Presence of virulence genes shown in blue, while absence in light green.

Article Snippet: Following the ISO/TS 13136:2012 method (ISO, 2012) each sponge was put into a sterile bag containing 90 ml of modified Tryptone Soya Broth (mTSB; Oxoid) added with novobiocin (Oxoid), homogenised in a stomacher blender for 2 min and incubated at 37 ◦C ± 1 ◦C for 18 to 24 h. After enrichment, a Real-Time PCR was conducted as described in the ISO method using the iQ-Check STEC VirX kit (Biorad, Hercules, CA, USA).

Techniques:

Journal: International journal of food microbiology

Article Title: Emerging of Shiga toxin-producing Escherichia coli O177:H11 and O177:H25 from cattle at slaughter in Italy.

doi: 10.1016/j.ijfoodmicro.2024.110846

Figure Lengend Snippet: Fig. 2. a) SNPs distance matrix results of the STEC O177:H25 strains using A2 as reference. b) Phylogenetic tree based on the maximum likelihood method of the STEC O177:H25 strains. The A2 isolate was used as outgroup to root the tree.

Article Snippet: Following the ISO/TS 13136:2012 method (ISO, 2012) each sponge was put into a sterile bag containing 90 ml of modified Tryptone Soya Broth (mTSB; Oxoid) added with novobiocin (Oxoid), homogenised in a stomacher blender for 2 min and incubated at 37 ◦C ± 1 ◦C for 18 to 24 h. After enrichment, a Real-Time PCR was conducted as described in the ISO method using the iQ-Check STEC VirX kit (Biorad, Hercules, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Enhanced Yield of Recombinant Proteins with Site-Specifically Incorporated Unnatural Amino Acids Using a Cell-Free Expression System

doi: 10.1371/journal.pone.0068363

Figure Lengend Snippet: Synthesis of WT GFP and the GFP Y39TAG mutant was performed using the RTS E. coli HY Kit, to which the corresponding plasmid (500 µg/mL), purified Mj TyrRS and cognate suppressor Mj tRNA CUA (tRNA) or T-stem modified tRNA CUA Opt (denoted as *) were added. (A) Expression of WT GFP and the GFP Y39TAG mutant in the presence of Mj TyrRS (300 µg/mL) and synthetic Mj tRNA CUA (60 µg/mL). The band at 28 kDa corresponds to full-length GFP. (B) Western blot analysis demonstrates enhanced GFP Y39TAG protein expression as a function of increased Mj TyrRS concentrations in a cell-free reaction medium supplied with Mj tRNA CUA (60 µg/mL – top panel and 450 µg/mL – bottom panel). ( C ) Dependence of GFP Y39TAG yield on the type and concentration of nonsense suppressor, as visualized by Western blot.

Article Snippet: Cell-free protein expression was performed using the RTS 100 E. coli HY Kit (5 PRIME, Hamburg, Germany) at 30°C for 6 h in 10 (for Western Blot) or in 50 μL (for protein purification and MS analysis) of reaction mixture.

Techniques: Mutagenesis, Plasmid Preparation, Purification, Modification, Expressing, Western Blot, Concentration Assay

Journal: Chinese Medicine

Article Title: Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure

doi: 10.1186/s13020-017-0131-3

Figure Lengend Snippet: Representative immunohistochemistry images ( a ) and quantitative analysis ( b , c , d ) of Bcl-2, Bax, and caspase 3 in histological sections from all experimental groups. Immunostaining ( brown ) are indicated by black arrows . For each animal, ten random high power fields (HPFs) from five sections were used for quantitative analysis. Each dot in b and c represents the median value across these HPFs. Bars and error bars are medians and quartiles, respectively. All statistical analyses were performed using the nonparametric Kruskal–Wallis test followed by Dunn’s post hoc test. NS not significant. Magnification ×100

Article Snippet: IHC kits for Bax (Catalog No.: BA0315-1), Bcl-2 (Catalog No.: BA0412), and caspase-3 (active form, Catalog No.: BA2142) were provided by Wuhan Boster Bio-engineering Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemistry, Immunostaining